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KMID : 0376219870240010037
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Chonnam Medical Journal
1987 Volume.24 No. 1 p.37 ~ p.48
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Antagonism of aminoglycoside antibiotics with rifampin in the activity against Staphylococcus aureus
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金種國/Kim, Jong-Kook
吳宗錫/安泰烋/Oh, Jong-Suk/Ahn, Tai-Hew
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Abstract
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Minimum inhibitory concentration(MIC) of rifampin(RF) and 5 kinds of aminoglycoside antibiotics, amikacin(AK), kanamycin(KM), tobramycin(TM), dibekacin(DK), and streptomycin(SM), were measured against 33 strains of Staphylococcus aureus.
Combined antibiotic activities of RF with each of the aminoglycoside antibiotics were then measured against each of the test strains, utilizing a modified method, i.e., fixed one-fourth MIC dilution of RF was mixed with serial two-fold dilutions of each aminoglycoside instead of mixing the serial two-fold dilutions of both drugs as in the conventional combined antibiotic test procedures.
The results obtained may be summerized as follows. In the preliminary drug susceptibility test, 24 strains were shown highly susceptible to (MIC< 0.0063μg/ml) while remaining 9 were highly resistant (MIC>50,ug/ml). The RF-resistant strains showed also a high resistance to each of the aminogly￢coside antibiotics in general except for SM to which 6 out of the 9 strains resistant to RF exhibited a high susceptibility. RF-susceptible strains, however, revealed a variety of attitude: half of the 24 strains were resistant to KM and SM, while all of them were susceptible to AK. It was interesting to notice that there was a similarity between TM and DK in the susceptibility pattern of the strains.
The combined antibiotic test revealed the general tendency that antagonism was dominant over synergiam. RF-AK showed antagonism in 20 strains out of 33 and only indifferent activity in the remaining ones; RF? KM exhibited indifference in 26, addition in 4, and antagonism in 3; RF-TM turned out to be indifference in 18, antagonism in 9, addition in 5, and synergism in 1; RF?DK showed indifference in 14, addition in 8, antagonism in 6, and synergism in 5; RF?SM revealed antagonism in 15, indifference in 12, addition in 3, and synergism in 3.
It was noteworthy that the degree of each tested strain´s resistance to in￢dividual drug had no effect on the combined antibiotic MIC in the present experiment, indicating the disappearance of the general tendency that the higher was the MIC of each antibiotic the lower was the combined MIC.
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